What is TAE 50X Buffer used for in molecular biology?

TAE 50X Buffer is primarily used in molecular biology applications, particularly in agarose gel electrophoresis. It serves as a running buffer for DNA and RNA samples, helping to maintain a stable pH and ionic strength during electrophoresis. The buffer's composition of Tris base, acetic acid, and EDTA provides an optimal environment for nucleic acids, enhancing their resolution and integrity during analysis. Researchers often use TAE for separating DNA fragments based on size, making it essential in applications such as cloning, sequencing, and genotyping.

How do you dilute TAE 50X Buffer for use?

To use TAE 50X Buffer, it must be diluted to a working concentration. You can achieve this by mixing 1 part of the 50X solution with 49 parts of distilled water, resulting in a 1X TAE Buffer ready for electrophoresis. This dilution ensures that the buffer provides the necessary conductivity and pH stability needed for optimal nucleic acid separation. It's important to prepare this solution fresh for best results, especially when working with sensitive samples that require precise conditions.

Is TAE Buffer suitable for RNA electrophoresis?

Yes, TAE Buffer is suitable for RNA electrophoresis. Its components help protect RNA from degradation during gel electrophoresis, making it a preferred choice for researchers analyzing RNA samples. The EDTA in TAE Buffer chelates divalent metal ions that could otherwise lead to RNA degradation, thus enhancing the stability of RNA during the process. This makes TAE an ideal buffer for applications like Northern blotting or RNA quality assessment through gel visualization.

Can TAE 50X Buffer be used for DNA preservation?

No, TAE 50X Buffer is not designed for DNA preservation in storage. While it is effective during electrophoresis, long-term storage of DNA should ideally involve buffers specifically formulated for preservation, such as TE buffer, which includes Tris and EDTA in a lower concentration more compatible for long-term storage. Utilizing TAE for preservation may lead to decreased DNA quality or integrity over time, as it is formulated for short-term use in electrophoresis applications.

What are the differences between TAE and TBE Buffers?

TAE and TBE Buffers differ primarily in their ionic strength and components. TAE Buffer is composed of Tris, acetic acid, and EDTA, while TBE includes Tris, boric acid, and EDTA. TAE is better for DNA analysis as it runs DNA gels slower and produces sharper bands, ideal for applications needing high resolution. Conversely, TBE has a higher buffering capacity and is preferred for separating smaller DNA fragments. The choice between them depends on the specific requirements of your experiment.

How should TAE 50X Buffer be stored for optimal use?

TAE 50X Buffer should be stored in a cool, dark place, ideally at 4°C to maintain its stability. Exposure to light and heat can degrade the buffer components, affecting its performance in experiments. Make sure to keep the container tightly closed to prevent evaporation and contamination. For best practices, it’s advisable to prepare only the necessary amounts, minimizing the frequency of opening the stock solution to maintain its integrity for electrophoresis.

Can TAE Buffer be reused after electrophoresis?

Reusing TAE Buffer from electrophoresis is generally not recommended. The buffer can accumulate impurities, nucleic acid residues, and other contaminants during use, which can adversely affect future experiments. Each time you run a gel, it is best to utilize fresh buffer to ensure consistent results and reliable data interpretation. This practice helps maintain the clarity of bands and the overall quality of your electrophoretic analysis.

What kind of samples can be run using TAE 50X Buffer?

TAE 50X Buffer is ideal for running a variety of nucleic acid samples, including plasmid DNA, genomic DNA, and RNA. Its formulation is particularly suited for larger DNA fragments, making it excellent for applications such as isolating and analyzing PCR products or restriction enzyme digests. Researchers often utilize it to achieve high resolution of these samples during gel electrophoresis, which is essential for downstream applications like cloning or sequencing.

What is the shelf life of TAE 50X Buffer?

The shelf life of TAE 50X Buffer is generally indicated on the label of the product, but when stored properly at 4°C, it can last for several months to even a few years. To ensure it remains effective, look for any signs of precipitation or discoloration, as these may indicate degradation. Regularly checking the buffer’s appearance and following the manufacturer's recommendations will help maintain its quality and effectiveness in your experiments.

Where can I buy TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter?

You can purchase TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter from Ubuy. Ubuy offers a variety of laboratory supplies, ensuring you have access to quality products for your research. Shopping on Ubuy allows you to get this versatile buffer delivered directly to your location while providing multiple options for securing your lab essentials.

Πώς να πραγματοποιήσετε αγορές TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 μέσω Διαδικτύου από το Ubuy;

Είναι εύκολο να ψωνίσετε TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 στο διαδίκτυο από το Ubuy.. Απλώς πρέπει να αναζητήσετε το προϊόν, να επιλέξετε τη μέθοδο αποστολής κατά την ολοκλήρωση της αγοράς και να το παραλάβετε στην τοποθεσία σας.

Είναι το TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 διαθέσιμο για διαδικτυακές αγορές σε Cyprus;

Ναι, στο Ubuy Cyprus αυτό το προϊόν είναι διαθέσιμο για αγορές σε λογική τιμή.. Το TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 δεν είναι διαθέσιμο τοπικά, αλλά μπορείτε να μας εμπιστευτείτε τις υπηρεσίες ταχείας αποστολής μας.

Πόσος χρόνος χρειάζεται για να λάβετε το προϊόν μετά την υποβολή της παραγγελίας;

Ο χρόνος παράδοσης του προϊόντος που παραγγείλατε ποικίλλει ανάλογα με το τι έχετε παραγγείλει και τη μέθοδο αποστολής που έχετε επιλέξει.. Ο εκτιμώμενος χρόνος παράδοσης αναφέρεται κατά τη διαδικασία του ταμείου, γι' αυτό να είστε ξέγνοιαστοι όταν ψωνίζετε.

RPI Εμφάνιση Πρωτότυπου

TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter

TRIS Acetate EDTA 50X Solution is a versatile solution commonly used in various laboratory applications.

RPI

Προϊόν #: 10628325

TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter

97% of respondents would recommend this to a friend

Προϊόν #: 10628325

€ 182

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from ΗΠΑ}

Διαθεσιμότητα : Σε απόθεμα

Εισήχθη από κατάστημα USA

QTY:

Παραγγείλτε τώρα και πάρτε το περίπου: Παρασκευή, Οκτώβριος 02

Secured transaction

Our Top Logistics Partners

TRIS Acetate EDTA 50X Solution is a versatile solution commonly used in various laboratory applications.

Εγγύηση U-Care:

Κανένα

Επιλέξτε ένα σχέδιο

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of ΗΠΑ store (110-120). Recommended power converters Αγορασε τωρα.

What Stands Out

High Concentration

This 50X TRIS Acetate EDTA solution allows for convenient dilution, saving time and reducing waste for laboratories or academic settings by providing a versatile buffer for various applications.

Versatile Applications

Ideal for molecular biology and biochemistry experiments, this buffer supports a wide range of procedures such as electrophoresis, gel preparation, and nucleic acid isolation, meeting diverse research needs.

Reliable Quality

Manufactured under stringent quality controls, this 1-liter buffer ensures consistent results in experiments, providing researchers with the confidence they need to achieve accurate and repeatable outcomes.

Λεπτομέρειες προιόντος

TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter
Sold for research or further manufacturing use only, not for food or drug use
CAS Number: 77-86-1; 64-19-7; 6381-92-6; 7732-18-5
Common Name: TRIS Acetate EDTA 50X Solution
Solution contains: Tris (hydroxymethyl) aminomethane: 2 M Acetic Acid: 1 M EDTA, Disodium Salt Dihydrate: 50 mM
Substance Name: TRIS Acetate EDTA 50X Solution
CAS Number(s): 77-86-1; 64-19-7; 6381-92-6; 7732-18-5

Product Technical Details

Product Dimensions	‎2.25 x 5.25 x 7.5 inches
Item Weight	‎1.19 pounds
Manufacturer	‎RPI, Corp.

Who Should Buy?

Molecular Biologists
Ideal for researchers conducting gel electrophoresis and nucleic acid studies utilizing TAE buffers for optimal results.
Educational Institutions
Perfect for laboratories in schools and universities that require reliable buffers for basic science experiments.
Researchers
Beneficial for scientists in genetics and biochemistry needing high-quality buffer solutions for experimentation and analysis.

Non-Scientific Users
Not suitable for individuals outside scientific fields, as it requires knowledge for proper application and usage.

ΠΕΡΙΓΡΑΦΗ ΤΟΥ ΠΡΟΪΟΝΤΟΣ

About This Item

Introducing the TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], the perfect solution for your laboratory needs. With its precise composition and high quality, this 1 liter bottle is a must-have for any researcher or scientist. The TAE 50X Buffer contains a unique blend of essential substances to ensure optimal performance. It is composed of 2 M Tris (hydroxymethyl) aminomethane, 1 M Acetic Acid, and 50 mM of EDTA, Disodium Salt Dihydrate.

These ingredients work together to provide the ideal environment for various laboratory applications. TRIS Acetate EDTA 50X Solution is widely used in molecular biology research, DNA sequencing, and gel electrophoresis. Its superior buffering capacity and excellent pH stability make it an essential component in many DNA and RNA extraction protocols. This solution ensures accurate and reproducible results, every time. When it comes to quality and reliability, TAE 50X Buffer is second to none.

We source our ingredients from trusted suppliers to guarantee the highest level of purity and consistency. Our solution is rigorously tested to meet industry standards, ensuring its superior performance and reliability. With its convenient 1 liter size, this TAE 50X Buffer provides ample supply for your laboratory needs. The bottle is designed for easy handling and storage, with a secure cap to prevent leakage and contamination.

Whether you are a seasoned scientist or a beginner in the field, this solution will provide you with the results you desire. Shop with confidence at Ubuy, your trusted e-commerce destination for TAE 50X Buffer and TRIS Acetate EDTA 50X Solution. Our competitive prices, fast shipping, and excellent customer service make us the preferred choice for researchers worldwide. Don't miss out on this essential laboratory solution, order your TAE 50X Buffer today and unlock the full potential of your research.

Έχετε κάποιο ερώτημα; Συνομιλήστε μαζί μας

Ερωτήσεις & Απαντήσεις Πελατών

ερώτηση: Πώς να πραγματοποιήσετε αγορές TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 μέσω Διαδικτύου από το Ubuy;

απάντηση: Είναι εύκολο να ψωνίσετε TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 στο διαδίκτυο από το Ubuy.. Απλώς πρέπει να αναζητήσετε το προϊόν, να επιλέξετε τη μέθοδο αποστολής κατά την ολοκλήρωση της αγοράς και να το παραλάβετε στην τοποθεσία σας.
ερώτηση: Είναι το TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 διαθέσιμο για διαδικτυακές αγορές σε Cyprus;

απάντηση: Ναι, στο Ubuy Cyprus αυτό το προϊόν είναι διαθέσιμο για αγορές σε λογική τιμή.. Το TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 δεν είναι διαθέσιμο τοπικά, αλλά μπορείτε να μας εμπιστευτείτε τις υπηρεσίες ταχείας αποστολής μας.
ερώτηση: Πόσος χρόνος χρειάζεται για να λάβετε το προϊόν μετά την υποβολή της παραγγελίας;

απάντηση: Ο χρόνος παράδοσης του προϊόντος που παραγγείλατε ποικίλλει ανάλογα με το τι έχετε παραγγείλει και τη μέθοδο αποστολής που έχετε επιλέξει.. Ο εκτιμώμενος χρόνος παράδοσης αναφέρεται κατά τη διαδικασία του ταμείου, γι' αυτό να είστε ξέγνοιαστοι όταν ψωνίζετε.

RPI Buffers Editorial Review

Δεν βρέθηκαν κριτικές σύνταξης

Customer Reviews & Ratings

5.0

1 βαθμολογίες πελατών

5 αστέρι

100%
4 αστέρι

0%
3 αστέρι

0%
2 αστέρι

0%
1 αστέρι

0%

Αξιολογήστε αυτό το προϊόν

Κοινοποιήστε τις σκέψεις σας με άλλους πελάτες

Πλεονεκτήματα

High Concentration
Easy to Dilute
Stable Formulation
Cost-Effective
Versatile Applications

Μειονεκτήματα

Requires careful handling

Product Price History

Σημαντικές πληροφορίες

Περιορισμοί : Για προϊόντα που αποστέλλονται διεθνώς, σημειώστε ότι τυχόν εγγύηση του κατασκευαστή ενδέχεται να μην είναι έγκυρη, οι επιλογές εξυπηρέτησης του κατασκευαστή ενδέχεται να μην είναι διαθέσιμες, τα εγχειρίδια, οι οδηγίες και οι προειδοποιήσεις ασφαλείας του προϊόντος ενδέχεται να μην είναι στη γλώσσα της χώρας προορισμού, τα προϊόντα (και τα συνοδευτικά υλικά) ενδέχεται να μην είναι σχεδιασμένα σύμφωνα με τα πρότυπα, τις προδιαγραφές και τις απαιτήσεις επισήμανσης της χώρας προορισμού και τα προϊόντα ενδέχεται να μην συμμορφώνονται με την τάση και άλλα ηλεκτρικά πρότυπα της χώρας προορισμού (απαιτώντας τη χρήση προσαρμογέα ή μετατροπέα, εάν χρειάζεται). Ο παραλήπτης είναι υπεύθυνος να διασφαλίσει ότι το προϊόν μπορεί να εισαχθεί νόμιμα στη χώρα προορισμού. Όταν παραγγέλνετε από το Ubuy ή τις θυγατρικές της, ο παραλήπτης είναι ο εισαγωγέας αρχείου και πρέπει να συμμορφώνεται με όλους τους νόμους και τους κανονισμούς της χώρας προορισμού.
Δεν είναι όλα τα προϊόντα που παρατίθενται στο Ubuy προς πώληση, καθώς το Ubuy είναι μια παγκόσμια μηχανή αναζήτησης. Τα προϊόντα υπόκεινται σε κανονισμούς εξαγωγής/εμπορίου.

€ 182

Παραγγείλτε τώρα και πάρτε το περίπου: Παρασκευή, Οκτώβριος 02

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter

€ 182

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Λεπτομέρειες προιόντος

Product Technical Details

Who Should Buy?

ΠΕΡΙΓΡΑΦΗ ΤΟΥ ΠΡΟΪΟΝΤΟΣ

About This Item

Ερωτήσεις & Απαντήσεις Πελατών

RPI Buffers Editorial Review

Customer Reviews & Ratings

Αξιολογήστε αυτό το προϊόν

Πλεονεκτήματα

Μειονεκτήματα

Product Price History

Σημαντικές πληροφορίες

€ 182

Ubuy Assurance

Fast
Shipping

Free
Return^*